Targeted Metagenomics using Short Fragment Sequencing
Characterize microbiome diversity
Metabarcoding has been an integral methodology for characterizing the diversity of microbiomes for decades; it can generate analyses of entire communities of bacteria or fungi in any complex sample. Like many metagenomic approaches, amplicon sequencing offers unique advantages for exploring and monitoring microbiomes.
A whole range of markers are available. For example, for bacteria, the 16S rRNA identity marker gene, which consists of ~1500 base pairs, is composed of 9 hypervariable regions (V1-V9) and is universally present in all prokaryotic organisms (Bacteria and Archaea). By selecting specific hypervariable regions for PCR amplification and sequencing, such as V1-V3 or V3-V4, METYS generates bacterial community profiles for comparative analysis across a wide range of matrices.
- Classify and monitor biodiversity
- Monitor microbiological contamination
- Development of pharmaceutical and agrochemical products
- Impact of industrial activities
- Microbial culture monitoring
- Water and environmental quality
- Biostimulants and biocontrol
What METYS offers
- AVITI (Element Biosciences)
- Expected sequence length: up to 300 bp (2 x 300 bp in pair-ends)
- 60Gb / flowcell (2 x 300 bp paired-end)
- Less than 2% error on sequences aligned with the control genome (PhiX)
- 2 lines per flowcell
- Multiplexing of up to 380 indexes in PCR product sequencing
- Interactive report, abundance tables and raw data
From nucleic acid extraction to bioinformatics and biostatistical analysis of data on a wide range of markers: 16S rRNA, 18S rRNA, ITS, 23S rRNA, genes (Tuf, GyrB, rbcL), or any other specific marker.
Comparison of MiSeq (Illumina) and AVITI (Element Biosciences) technologies on microbiomes
