Targeted Metagenomics using Long Fragment Sequencing
Sequencing of Full-Length 16s amplicons in PACBIO
The 16S rRNA gene is ~1500 base pairs long, composed of 9 hypervariable regions (V1-V9) and universally present in all prokaryotic organisms (Bacteria and Archaea). Traditional targeted metagenomic analysis (short fragment) consists of selecting and sequencing hypervariable regions (e.g. 16S V1-V3 or V3-V4) with the aim of providing microbial identification mainly at genus level.
The recent arrival of third-generation sequencers has opened up new fields of exploration. Sequencing with PacBio technology delivers highly accurate long reads, covering all 1500 bp of the 16S rRNA gene, improving the resolution of species identification in complex microbial ecosystems.
- Characterize and monitor biodiversity
- Monitor microbiological contamination
- Development of pharmaceutical and agrochemical products
- Impact of industrial activities
- Microbial culture monitoring
- Water and environmental quality
- Biostimulants and biocontrol
What METYS offers
- PacBio Sequel II sequencer
- Sequence length: 1500 pb
- High reading accuracy with PacBio HiFi Reads
- Multiplexing of up to 96 samples per sequencing run
- Approx. 10K-30K HiFi reads per sample
- Applicable to various microbial community studies
- Interactive report, abundance tables and raw data
From nucleic acid extraction to bioinformatics and biostatistical analysis of data.