Shotgun Metagenomics

The complete genetic information of a sample

Shotgun metagenomics targets all the microbial genetic information contained in a sample, enabling the functional and taxonomic characterization of microbial communities. All bacteria, viruses, fungi and other microbial domains are identified by a single sequence. Unlike metabarcoding, Shotgun metagenomics is based on all areas of the genome, enabling precise taxonomic identification down to species level. It therefore provides a comprehensive overview of the microbiome’s composition and diversity of functions.

Characterize all microorganisms, such as viruses, by sequencing with universal primers
Combine taxonomy and function of microbial species
Obtain relative abundance levels of microbial species that are difficult or impossible to grow in the laboratory
Analyze the relative abundance of microbial species in the natural habitat
Profile the functional characteristics of a particular microbiome
Monitor changes in microbial community composition under variable environmental conditions
Observe changes in the relative abundance of microorganisms under varying conditions or stresses
Discover new biomarkers for diversity or function quantification

The Metys GeT-IT team works with a wide variety of samples, including: Water, air, soil, plants, seeds, food matrices, microbial consortia, swabs (skin, environmental/surface), previously extracted DNA.

Our Tools

Our Deliverables

Raw data : fasta and fasta.gz formats
Abundance tables that combine samples, taxonomy and abundance for each OTU to generate figures and interpret data yourself: Excel format
Diversity indices and rarefaction curves
An interactive report to export graphs and tables according to your needs: .html format

KEGG annotation tables (.tsv) and graphs (.png)
Correlation plots (.png)

Antibiotic resistance and virulence tables (.tsv) and graphs (.png)
Correlation plots (.png)